24109 ESTs
29357 Nucleotides (mRNA)
Combined
CDHIT EST-
running through CD-HIT_EST
./cd-hit-est -i /Volumes/Bay4\ scratch/temp/Haliotis_NCBI_combined.fasta -o /Volumes/Bay4\ scratch/temp/Haliotis_comboNCBI_cdhit -M 2500
robertsmac:cd-hit-v4.5.4-2011-03-07 sr320$ ./cd-hit-est -i /Volumes/Bay4\ scratch/temp/Haliotis_NCBI_combined.fasta -o /Volumes/Bay4\ scratch/temp/Haliotis_comboNCBI_cdhit -M 2500
================================================================
Program: CD-HIT, V4.5.4, Feb 23 2012, 11:03:06
Command: ./cd-hit-est -i
/Volumes/Bay4 scratch/temp/Haliotis_NCBI_combined.fasta
-o /Volumes/Bay4 scratch/temp/Haliotis_comboNCBI_cdhit
-M 2500
Started: Fri Apr 20 15:14:24 2012
================================================================
Output
----------------------------------------------------------------
total seq: 53466
longest and shortest : 11166 and 28
Total letters: 24083836
Sequences have been sorted
Approximated minimal memory consumption:
Sequence : 30M
Buffer : 1 X 15M = 15M
Table : 1 X 17M = 17M
Miscellaneous : 4M
Total : 68M
Table limit with the given memory limit:
Max number of representatives: 4194304
Max number of word counting entries: 303948411
Reduced it down to
40521 sequences
----
18,444 are larger that 400bp
Blasting on BFX………..
OUTPUT